Description
Product Usage Information
Western Blotting: 1:1000
IHC Leica Bond: 1:250 - 1:1000
Immunohistochemistry (Paraffin): 1:250 - 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
MAOA (E2H1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAOA protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro243 of human MAOA protein.
Background
Monoamine oxidase A (MAOA), a mitochondrial enzyme, catalyzes the degradation of monoamines. Studies show that increased expression of MAOA promotes prostate cancer metastasis by activating the Shh-IL6-RANKL signaling network (1). In addition, MAOA expression in adipose tissue macrophages is upregulated in aging, leading to the reduction of noradrenaline availability in adipose tissue. Decreased levels of noradrenaline contribute to the aging-related decline of catecholamine-induced lipolysis in adipocytes (2). Furthermore, sympathetic neuron-associated macrophages import and degrade norepinephrine via the transporter SLC6A2 and MAOA, respectively, and thus contribute to obesity (3).
Alternate Names
Amine oxidase [flavin-containing] A; AOFA; BRNRS; MAO-A; MAOA; Monoamine oxidase; monoamine oxidase A; Monoamine oxidase type A
