Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
UBXN1 (E5B5J) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBXN1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu101 of human UBXN1 protein.
Background
Ubiquitin regulatory X (UBX) domain-containing protein 1 (UBXN1) is a modular ubiquitin binding protein that consists of three domains: an N-terminal ubiquitin-associated (UBA) domain, a central coiled-coil domain, and a C-terminal UBX domain (1). UBXN1 has been shown to modulate the host innate immune response to viral infection. Indeed, research studies have demonstrated that UBXN1 retrains the type I interferon response induced by RNA viruses by binding to MAVS and compromising its oligomerization (2). UBXN1 has also been shown to negatively regulate NF-κB activation in response to proinflammatory cytokines, a finding which may have implications for antiviral signaling cascades (3). In addition to regulating antiviral signaling cascades, research has shown that UBXN1 participates in protein quality control by interacting with p97/VCP (4).
Alternate Names
2B28; LOC51035; SAKS1; SAPK substrate protein 1; UBA/UBX 33.3 kDa protein; UBX domain protein 1; UBX domain-containing protein 1; UBXD10; UBXN1
