Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:200 - 1:800
Immunofluorescence (Frozen): 1:200 - 1:400
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44550 .
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total desmoplakin protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human desmoplakin protein.
Background
Desmosomes are a class of intracellular junctions that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).
Alternate Names
250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; Desmoplakin; desmoplakin I; desmoplakin II; DESP; DP; DPI; DPII; DSP
