Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
MAPKAPK-5 (D70A10) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAPKAPK-5 protein. This antibody does not cross-react with MAPKAPK-2 or MAPKAPK-3.
Species Reactivity: Human, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly367 of human MAPKAPK-5 protein.
Background
MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).
Alternate Names
MAP kinase-activated protein kinase 5; MAPK activated protein kinase 5; MAPK-activated protein kinase 5; MAPK5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MK-5; MK5; p38-regulated/activated protein kinase; PRAK
