Description
Protocol
Available protocols: ELISA+
Specificity / Sensitivity
The FastScan™ Total GSK-3 alpha ELISA Kit detects endogenous levels of GSK-3α, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity: Human, Mouse, Rat, Monkey
Background
Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in and is a component of the Wnt signaling pathway required for and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
Alternate Names
DKFZp686D0638; glycogen synthase kinase 3 alpha; Glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK3A; GSK3alpha; Serine/threonine-protein kinase GSK3A
