Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:100 - 1:400
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunohistochemistry (Paraffin)
Specificity / Sensitivity
CD11b/ITGAM (E4K8C) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser832 of mouse CD11b/ITGAM protein.
Background
Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).
Alternate Names
CD11 antigen-like family member B; Cd11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; integrin alpha M; Integrin alpha-M; ITAM; Itgam; Leukocyte adhesion receptor MO1; Ly-4; Ly-40; Mac-; Mac-1; Mac-1 alpha; Mac-1a; MAC1; macrophage antigen alpha
