Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Frozen): 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)
Specificity / Sensitivity
mGluR2 (D7D8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total mGluR2 protein. This antibody may cross-react with mGluR3.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular loops of mouse mGluR2 protein.
Background
Metabotropic glutamate receptor 2 (mGluR2) is a class C G protein-coupled receptor for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). While mGluR1 and mGluR5 activate phospholipase C, mGluR2, mGluR3, mGluR4, and mGluR6 are coupled to the inhibitory G protein Gα(i/o) and inhibit adenylyl cyclase (AC) activity (1). Research studies have suggested that mGluR2/3 receptors may be potential targets for the treatment of Schizophrenia (2). Furthermore, mGluR2 interacts with the 5HT2A serotonin receptor to form a hetero-complex in the brain. This complex is a potential pharmacological target for hallucinogenic drugs (3,4).
Alternate Names
GLUR2; glutamate metabotropic receptor 2; glutamate receptor homolog; glutamate receptor, metabotropic 2; GPRC1B; GRM2; Metabotropic glutamate receptor 2; mGlu2; MGLUR2
