Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
MICB (E4I1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total MICB protein. This antibody does not cross-react with MICA protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus (extracellular region) of human MICB protein.
Background
Natural killer group 2D (NKG2D) is an activating receptor expressed on NK and some T cells. It recognizes a variety of ligands that belong to the non-classical MHC class I family, including MICA, MICB, ULBP1-6 in human and Mult1, H60 (a, b, and c), and Rae-1 (α-ε) in mice. These ligand proteins are not expressed in most tissues under normal conditions, but are expressed in cells under stress, such as viral infection and malignant transformation. Engagement of NKG2D by these ligands activates cytolytic activity of NK and T cells against target cells, providing immunosurveillance. Some of these ligands are polymorphic. There are more than 100 different alleles for MICA, 40 for MICB, and around 20 for ULBPs (1-4). In addition, some of these ligands can be proteolytically cleaved from the cell surface to become soluble forms. The interaction of soluble ligands with NKG2D results in endocytosis and degradation of ligand-receptor complexes, therefore suppressing NKG2D-mediated immunosurveillance (5,6). However, recent data also demonstrated that soluble ligands can promote NK cell activation and tumor rejection (7,8).
Alternate Names
MHC class I chain-related molecule B; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I molecule; MHC class I polypeptide-related sequence B; MHC class I-like molecule PERB11.2-IMX; MIC-B; MICB; PERB11.2; stress inducible class I homolog
