Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
IHC Leica Bond: 1:50 - 1:200
Immunohistochemistry (Paraffin): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 92841 .
Protocol
Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
CD31 (PECAM-1) (D8V9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD31 (PECAM-1) protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala451 of mouse CD31 (PECAM-1) protein.
Background
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
Alternate Names
adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; FLJ58394; GPIIA'; PECA1; PECAM-1; PECAM-1, CD31/EndoCAM; PECAM1; platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule
