Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
METTL5 (F4E9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total METTL5 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu13 of human METTL5 protein.
Background
Methyltransferase-like protein 5 (METTL5) forms a stable complex with TRMT112 in order to catalyze N6-methyladenosine on the 18S rRNA at position 1832 (1). METTL5 can affect the global translation rate, pluripotency, differentiation, and neural development (2-4). High expression of METTL5 has been described in lung adenocarcinoma where it contributes to epithelial-mesenchymal transition (5,6). In pancreatic cancer, METTL5 promotes Myc translation to drive progression (6).
Alternate Names
FLJ10459; HSPC133; methyltransferase like 5; Methyltransferase-like protein 5; METL5; METTL5; MRT72; rRNA N6-adenosine-methyltransferase METTL5
