Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ELF4 (F8H6X) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELF4 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ELF4 protein.
Background
ETS-related transcription factor Elf-4 (ELF4), also known as myeloid Elf-1-like factor (MEF), is a member of the ETS-family of transcription factors, all containing a well-conserved ETS DNA-binding domain that recognizes a core consensus sequence defined as 5-'GGAA' (1,2). In addition to being a transcriptional activator, ELF4 also plays many roles within the immune system, such as regulating natural killer (NK) cell development and perforin expression, CD8 T cell proliferation, anti-viral immunity through type I interferon, and TH17 cell differentiation (1-6). Lack of in humans has been associated with deficiency in ELF4, X-linked (DEX), a monogenic autoinflammatory disease (mAID) (5,6). In mouse models, KO macrophages failed to sustain the transcription of key anti-inflammatory genes and instead upregulated proinflammatory genes (5).
Alternate Names
E74 like ETS transcription factor 4; E74-like factor 4; E74-like factor 4 (ets domain transcription factor); ELF4; ELFR; ETS-related transcription factor Elf-4; MEF; Myeloid Elf-1-like factor
