Description
Storage
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Protocol
Available protocols: ELISA+
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan
Species Reactivity: Human, Mouse, Rat, Hamster, Monkey
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Alternate Names
cell based assay; cell-based assay; cytoskeleton; housekeeping; loading control; microtubules; normalization; sandwich ELISA; screening; total protein; tubulin
