Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:1600 - 1:3200
Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Flow Cytometry (Live): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 35621 .
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
PILRA (E6H7K) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse PILRA protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse PILRA protein.
Background
Paired immunoglobulin-like type 2 receptor alpha (PILRA) is an inhibitory surface receptor expressed by myeloid cells and other tissues, including microglia in the central nervous system, regulating immune cells and inflammation (1). It functions by recruiting cytoplasmic phosphatases like PTPN6/SHP-1 and PTPN11/SHP-2, blocking downstream signal transduction via their SH2 domains (2). Furthermore, PILRA plays a role as an entry co-receptor for herpes simplex virus-1 via binding of viral glycoprotein B1 (3). More recently, mutations in the gene have been implicated in Alzheimer's disease (AD) (1,4). Recent large-scale GWAS have linked the risk of developing late-onset AD in humans harboring rare variants of microglial immunoreceptors such as TREM2, CD33, and PILRA (5).
Alternate Names
AV021745; Cell surface receptor FDF03; FDF03; Inhibitory receptor PILR-alpha; OTTMUSP00000026272; paired immunoglobin-like type 2 receptor alpha; Paired immunoglobulin-like type 2 receptor alpha; Pilra
