Description
Storage
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
Protocol
Available protocols: ELISA+
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan
Species Reactivity: Human, Dog
Background
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Alternate Names
cell based assay; cell-based assay; protein tyrosine kinase; receptor tyrosine kinase; sandwich ELISA; screening; Src
