Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
LXR-alpha (E3D9B) Rabbit Monoclonal Antibody recognizes overexpressed levels of total LXR-α protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LXR-α protein.
Background
Liver X receptors LXR-α and LXR-β are nuclear hormone receptor superfamily members responsible for regulating expression of target genes that control cholesterol transport and metabolism (1). When bound by the oxidized derivatives of cholesterol (oxysterols), activated LXR receptors function as sterol sensors to regulate transcription of the genes involved in the cholesterol homeostasis (1,2). The LXR-α protein is expressed at high levels in rat liver, kidney, intestine, adipose, and spleen; LXR-β is more ubiquitously expressed within rat tissues (1,3). Research studies indicate that glucose binds and upregulates the transcriptional activity of LXR-α and LXR-β (4). LXR-α and LXR-β are putative glucose sensors that integrate glucose metabolism and fatty acid biosynthesis in the liver (4). Additional studies show that female mice deficient in LXR-β develop gallbladder cancer (5). In addition, LXR-β plays a role in protecting dopaminergic neurons in a Parkinson's disease model (6).
Alternate Names
liver X nuclear receptor alpha variant 1; Liver X receptor alpha; liver X receptor-alpha; liver X receptor, alpha; LXR-a; LXR-alpha; LXRA; NR1H3; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; Oxysterols receptor LXR-alpha; RLD-1
