Description
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 .
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Chromatin IP: 1:100
CUT&RUN: 1:50
CUT&Tag: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24748 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN, CUT&Tag
Specificity / Sensitivity
GATA-2 (E9T6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total GATA-2 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GATA-2 protein.
Background
The zinc finger transcription factor GATA-2 is widely expressed and plays an essential role in many developmental processes (1). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (2). GATA-2 also inhibits the differentiation of white (3) and brown adipocytes (4) and has been shown to suppress the proliferation of neuronal progenitor cells (5).
Alternate Names
DCML; Endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA-binding protein 2; GATA2; IMD21; MGC2306; MONOMAC; NFE1B
