Description
Product Usage Information
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin/FN1 protein.
Background
Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6).
Alternate Names
Anastellin; CIG; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; epididymis secretory sperm binding protein; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF; Ugl-Y1; Ugl-Y2; Ugl-Y3
