Description
Storage
Supplied as a lyophilized product. Keep at -20°C for short-term storage or at -80°C for longer periods. Avoid repeated freeze-thaw cycles. Reconstitution information: The recommended final concentration for this positive control lysate is 0.5 mg/ml. Reconstitute the lyophilized positive control with 224 μL of dH 2 O to create a 3.0 mg/mL lysate solution. Dilute the sample with 1.0 mL 1X cell lysis buffer (#9803) or a sample diluent supplied with one of our PathScan® Sandwich ELISA kits. Keep on ice. Use 100 μL samples in each well of a 96-well plate.
Background
PathScan Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan Sandwich ELISA Control Phospho Cell Extracts II can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.
