Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Hip (10B7-C3) Mouse Monoclonal Antibody detects endogenous levels of total Hip (Hsc70-interacting) protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to rat Hip protein.
Background
Hip (HSP70-interacting protein), also known as ST13 (suppression of tumorigenicity protein 13), is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1,2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).
Alternate Names
AAG2; aging-associated protein 2; F10A1; FAM10A1; FAM10A4; FLJ27260; heat shock 70kD protein binding protein; HIP; HOP; Hsc70-interacting protein; Hsp70-interacting protein; HSPABP; HSPABP1; MGC129952; P48; PRO0786; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; SNC6; ST13; ST13 Hsp70 interacting protein; suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein); Suppression of tumorigenicity 13 protein; testis secretory sperm-binding protein Li 233m
