Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
DAZL Antibody recognizes endogenous levels of total DAZL protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DAZL protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The human (Deleted in Azoospermia) gene family contains at least three members that encode RNA-binding proteins with a common RNA-recognition motif (1). An autosomal homolog of DAZ, DAZL (DAZ-like), is specifically expressed in germ cells and is essential for the specification of the germ cell lineage during embryogenesis and during gametogenesis in adults of both sexes (2,3). DAZL may function by directly recruiting poly(A)-binding proteins (PABPs) in order to activate silent mRNAs during germ cell development (2). Deletions encompassing the Y chromosomal genes are the most common molecularly defined cause of infertility in humans (4,5).
Alternate Names
DAZ homolog; DAZ-like autosomal; DAZH; DAZL; DAZL1; DAZLA; deleted in azoospermia like; Deleted in azoospermia-like; Deleted in azoospermia-like 1; deleted in azoospermia-like autosomal; germline specific RNA binding protein; MGC26406; spermatogenesis gene on the Y-like autosomal; SPGY-like-autosomal; SPGYLA; testis secretory sperm-binding protein Li 204a
