Description
Product Usage Information
For more information about the RBP-eCLIP service please visit Eclipsebio .
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100 - 1:400
Immunofluorescence (Immunocytochemistry): 1:100 - 1:200
eCLIP: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
TFAM (D5C8) Rabbit Monoclonal Antibody recognizes endogenous levels of total TFAM protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val225 of human TFAM protein.
Background
TFAM (Transcription Factor A, Mitochondrial; aka TCF6) is a member of the high-mobility group (HMG) proteins because it contains two HMG boxes. TFAM is a transcription factor for mitochondrial DNA (mtDNA), and enhances mtDNA transcription in a promoter-specific fashion in the presence of mitochondrial RNA polymerase and transcription factor B (1). Because the majority of ATP production depends on the mitochondrial respiratory chain, maintenance of the mitochondrial genome is critical for normal health. TFAM plays an essential role in the maintenance of mtDNA and thus, ATP production (2). TFAM binds to mtDNA both nonspecifically and in a sequence-specific manner. It is known to have a dual effect on mtDNA: protection of mtDNA and initiation of transcription from mtDNA (3). TFAM attenuates age-dependent impairment of the brain by preventing oxidative stress and mitochondrial dysfunctions in microglia (4).
Alternate Names
Mitochondrial transcription factor 1; mitochondrial transcription factor A; MTDPS15; MtTF1; mtTFA; TCF-6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6; transcription factor 6-like 1; Transcription factor 6-like 2; Transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; Transcription factor A, mitochondrial
