Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
LINGO-1 (E5G3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total LINGO-1 protein. This antibody does not cross-react with other LINGO proteins.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LINGO-1 protein.
Background
Leucine-rich repeat and immunoglobulin domain-containing protein 1 (LINGO-1) is a potent negative modulator of neuronal processes, including neuronal survival, axonal integrity, oligodendrocyte differentiation, and myelination (1-5). LINGO-1, Nogo receptor (NgR), and p75 neurotrophin receptor (p75NTR), or TNF receptor orphan Y (TROY) form a tripartite receptor complex, which activates RhoA/ROCK signaling and is responsible for the inhibition effect of myelin- associated factors (6,7). LINGO-1 is abundantly expressed in the brain and is implicated in various neurodegenerative disorders, such as Essential tremor, multiple sclerosis, and Parkinson's disease (8-11). Recently, LINGO-1 was reported to bind directly to amyloid precursor protein (APP), promoting its degradation through lysosomal proteolysis (12). This research study implicated that LINGO-1 plays a critical role in the pathophysiology of Alzheimer's disease.
Alternate Names
FLJ14594; LERN1; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat and immunoglobulin domain-containing protein 1; Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1; leucine-rich repeat neuronal 6A; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LIGO1; LINGO1; LRRN6A; MGC17422; MRT64; UNQ201
