Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
STAP2 Antibody recognizes endogenous levels of total STAP2 protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human STAP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
STAP2 (signal-transducing adaptor protein 2, BRK substrate, BKS) is an adaptor protein expressed in various tissues and cell lines (1). The protein sequence of STAP2 contains a PH domain, an SH2 like domain, a proline rich region, and a YXXQ motif for interaction with various signaling molecules. (2). In the immune system, STAP2 plays important roles in cytokine signaling, macrophage activation, T cell migration, and motility via interaction with, and regulation of, signaling molecules such as STAT3, STAT5, IKKα/β, CSF1R, VAV, FAK, and Fas ligand. In leukemia, prostate cancer, breast cancer, and melanoma, STAP2 has been shown to promote tumor growth and metastasis via stabilization and activation of signaling molecules, including BCR/ABL, EGFR, BRK, STATs, and tyrosinase (3-6).
Alternate Names
BKS; Breast tumor kinase substrate; brk kinase substrate; BRK substrate; FLJ20234; signal transducing adaptor family member 2; Signal-transducing adaptor protein 2; signal-transducing adaptor protein-2; STAP-2; STAP2
