Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
MPP6/MPHOSPH6 (F3T9S) Rabbit Monoclonal Antibody recognizes endogenous levels of total MPP6/MPHOSPH6 protein. This antibody detects 52 and 54 kDa bands of unknown origin.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala151 of human MPP6/MPHOSPH6 protein.
Background
M-phase phosphoprotein 6 (MPP6 or MPHOSPH6) is a nuclear exosome protein that binds to pre-rRNAs to help facilitate the formation of mature 5.8S rRNA (1-3). MPP6, along with other exosome cofactors, can recruit the RNA helicase MTR4 to facilitate decay by unwinding RNA in order for it to enter the exosome core (4-6). MPP6 and YTHDC1 have been shown to play a role in IgH DNA recombination through an m6A-dependent mechanism (7).
Alternate Names
M-phase phosphoprotein 6; MPH6; MPHOSPH6; MPP; MPP-6; MPP6
