Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ERp29 (E6A1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ERp29 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly236 of human ERp29 protein.
Background
Endoplasmic reticulum protein 29 (ERp29) is a broadly expressed chaperone protein with roles in protein folding, trafficking, and secretion (1). ERp29 contributes to GAP junction formation by stabilizing monomeric connexin 43, preventing premature oligomerization in the endoplasmic reticulum and allowing for efficient transport to the plasma membrane (2). ERp29 is thought to contribute to tumorigenesis in some human cancers (3), but acts as a tumor suppressor in gastric cancer (4).
Alternate Names
C12orf8; endoplasmic reticulum lumenal protein ERp28; endoplasmic reticulum protein 29; Endoplasmic reticulum resident protein 28; Endoplasmic reticulum resident protein 29; Endoplasmic reticulum resident protein 31; epididymis secretory protein Li 107; ERP28; ERP29; ERp31; HEL-S-107; PDI-DB; PDIA9; protein disulfide isomerase family A, member 9
