Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
DYNLL1/LC8 (E6W7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DYNLL1/LC8 protein. This antibody cross-reacts with DYNLL2.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala28 of human DYNLL1/LC8 protein.
Background
Hub proteins are important components of protein interaction networks, binding to a wide variety of proteins, and often driving dimerization (1). The dynein light chain LC8 family members, DYNLL1 and DYNLL2, are conserved, ubiquitously expressed hub proteins that interact with a diverse group of proteins, including molecular motor proteins. LC8 family member protein interactions regulate multiple cellular processes (2). Loss of DYNLL1 in BRCA1-mutant cancer cells restores homologous recombination by allowing DNA end resection, conferring resistance to platinum and PARP inhibitor therapies (3). Â DYNLL1 regulation of DNA repair pathways likely occurs through its interaction with end resection machinery and 53BP1 (3,4). Upregulation of DYNLL1 has been associated with chemoresistance and progression of human cancer (5-7).
Alternate Names
8 kDa dynein light chain; cytoplasmic dynein light polypeptide; DLC1; DLC8; DNCL1; DNCLC1; DYL1; Dynein light chain 1, cytoplasmic; Dynein light chain LC8-type 1; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1; DYNLL1; HDLC1; LC8; LC8a; MGC126137; MGC126138; PIN; Protein inhibitor of neuronal nitric oxide synthase
