Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
hnRNP E2/PCBP2 (D1S5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP E2/PCBP2 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu243 of human hnRNP E2/PCBP2 protein.
Background
PCBP2 (also known as hnRNP E2) is an RNA-binding protein that interacts in a sequence-specific fashion with single-stranded poly (rC). Through their poly(rC)-binding ability, PCBPs regulate mRNA stability and translation (1,2). PCBP2 is an iron chaperone; it delivers iron to ferritin for storage and mediates metalation of certain iron-containing proteins (3-5). PCBP2 interacts with the iron importer DMT1 (divalent metal transporter 1) and the iron exporter FPN1 (ferroportin 1) and regulates their activities (6,7). PCBP2 is induced by viral infection and targets MAVS for polyubiquitination and degradation (8-10). Recent reports demonstrate that it is involved in Hippo signaling, miRNA processing, immune suppression, and cancer (11-15) .
Alternate Names
Alpha-CP2; epididymis secretory sperm binding protein; Heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; hnRNP-E2; HNRNPE2; HNRPE2; MGC110998; PCBP2; poly(rC) binding protein 2; Poly(rC)-binding protein 2
