Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
PEX5 (D7V4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PEX5 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe326 of human PEX5 protein.
Background
Peroxin-5 (PEX5) is the shuttle receptor that delivers proteins to peroxisomes (1). In the cytosol, PEX5 binds to the peroxisomal targeting signal 1 (PTS1), a short peptide sequence present at the extreme C termini of newly synthesized peroxisomal matrix proteins. The PEX5-cargo complex interacts with the peroxisomal docking/translocation machinery on the peroxisomal membrane, where the cargo is released into the organelle matrix. During this process, PEX5 is monoubiquitinated at a conserved cysteine residue, and the ubiquitin-PEX5 conjugate is released from the organelle into the cytosol, where PEX5 is deubiquitinated and ready the next round of targeting (2,3).
Alternate Names
FLJ50634; FLJ50721; FLJ51948; PBD2A; PBD2B; Peroxin-5; peroxisomal biogenesis factor 5; Peroxisomal C-terminal targeting signal import receptor; peroxisomal import receptor 5; peroxisomal targeting signal 1 (SKL type) receptor; Peroxisomal targeting signal 1 receptor; peroxisomal targeting signal import receptor; peroxisomal targeting signal receptor 1; Peroxisome receptor 1; PEX5; PTS1 receptor; PTS1-BP; PTS1R; PXR1; RCDP5
