Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Galectin-7/LGALS7 (F9K7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total galectin-7/LGALS7 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human galectin-7/LGALS7 protein.
Background
Galectin-7 belongs to the galectin family of carbohydrate-binding proteins, which specifically binds β-galactose-containing glycoconjugates and shares primary structural homology in the carbohydrate-recognition domain (CRD). Galectin-7 is a prototype galectin, containing a single CRD and forming homodimers (1). Galectin-7 is mainly expressed in stratified epithelial cells, where it contributes to tissue differentiation and development, and plays a crucial role in the re-epithelialization process of corneal or epidermal wounds induced by UV irradiation (2,3). The protein functions by stabilizing E-cadherin on the cell surface (4) and intracellularly activating JNK to induce pro-apoptotic caspase activity and cytochrome C release (5). Elevated galectin-7 expression is associated with many carcinomas. It promotes cancer migration and metastasis by activating ERK and JNK signaling, increasing MMP-9 and MMP-2 expression (6,7).
Alternate Names
Gal-7; GAL7; galectin 7B; Galectin-7; galectin-7B; HKL-14; keratinocyte lectin 14; lectin galactoside-binding soluble 7; lectin, galactoside-binding, soluble, 7B; LEG7; LGALS7; LGALS7B; MGC75149; MGC88745; p53-induced gene 1 protein; p53-induced protein 1; PI7; PIG1
