Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Prdx1 (D5G12) Rabbit Monoclonal Antibody recognizes endogenous levels of total Prdx1 protein. This antibody does not cross-react with Prdx2 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg110 of human Prdx1 protein.
Background
Prdx1 belongs to a family of non-seleno peroxidases that function as H O scavengers. All 6 Prdx isoforms share a conserved N-terminal cysteine (Cys51) that is oxidized by H O to form cysteine-sulfenic acid (Cys51-SOH) and, in turn, reacts with Cys172-SH of another Prdx protein, forming a disulfide dimer and protecting it from degradation (1-3). Abnormally high levels of H O cause Prdx1 to form an oligomeric chaperone that loses its peroxidase activity (4). Prdx family members have been reported to bind to JNK and c-Abl and regulate their kinase activity (5,6). Prdx1 was shown to bind to PTEN and regulate its phosphatase activity in conditions of mild or no cellular stress, hence preventing Akt-driven transformation by protecting PTEN from oxidation-induced inactivation (7).
Alternate Names
epididymis secretory sperm binding protein; MSP23; Natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEF-A; NKEFA; PAG; PAGA; PAGB; peroxiredoxin 1; Peroxiredoxin-1; PRDX1; proliferation-associated gene A; Proliferation-associated gene protein; PRX1; PRXI; TDPX2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; Thioredoxin-dependent peroxiredoxin 1
