Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Stargazin (D33G3) Rabbit Monoclonal Antibody recognizes endogenous levels of total stargazin protein.
Species Reactivity: Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala210 of human stargazin protein.
Background
Stargazin is a four-pass transmembrane protein related to VDCC (voltage dependent calcium channel) γ subunits and part of the TARP (transmembrane AMPA receptor regulatory protein) family of proteins. TARP proteins can form a complex with AMPA receptors (GluR1-4) and serve as integral auxiliary subunits (1-6). Interactions between stargazin and AMPA receptors are implicated in regulation of receptor surface expression, synaptic clustering and recycling, as well as increased receptor responsiveness to glutamate (1,2,5,6). Stargazin may play a role in the molecular mechanism of AMPAR-mediated inflammatory pain by taking part in signaling pathways that relay pain in the spinal cord (5). Because the protein also modulates the pharmacology of AMPA receptors, it enhances the effects of AMPAR potentiators that have therapeutic potential for a number of mental and neurodegenerative diseases (6). The carboxy terminus of the stargazin protein interacts with the PDZ domains of PSD95 and other membrane-associated guanylate kinase (MAGUK) family members, and together traffic AMPA receptors to the cell surface membrane, anchoring them to the postsynaptic site (1,7). Phosphorylation of stargazin by PKA on Thr321 inhibits this binding (3).
Alternate Names
CACNG2; calcium channel, voltage-dependent, gamma subunit 2; calcium voltage-gated channel auxiliary subunit gamma 2; CCG2; MGC138502; MGC138504; MRD10; Neuronal voltage-gated calcium channel gamma-2 subunit; stargazin; TARP gamma-2; Transmembrane AMPAR regulatory protein gamma-2; Voltage-dependent calcium channel gamma-2 subunit
