Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
DFCP1 (E9R6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total DFCP1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val451 of human DFCP1 protein.
Background
Double FYVE-containing protein 1 (DFCP1; gene name ZFYVE1), which was identified from a human bone marrow library, contains two carboxyl terminal FYVE domains that function as binding sites for phosphatidylinositol 3-phosphate (PI3P) (1). PI3P generated predominantly by the class III PI3-kinase VPS34 plays a key role in membrane trafficking as well as autophagy (2,3). DFCP1 is primarily localized to the Golgi and endoplasmic reticulum (ER) (4,5). However, during autophagy DFCP1 re-localizes to subdomains of the ER, the omegasome, which become the sites for autophagosome formation (6,7).
Alternate Names
DFCP1; Double FYVE-containing protein 1; KIAA1589; phosphoinositide-binding protein SR3; PPP1R172; protein phosphatase 1, regulatory subunit 172; SR3; TAFF1; Tandem FYVE fingers-1; tandem FYVE fingers-1 protein; ZFYV1; ZFYVE1; Zinc finger FYVE domain-containing protein 1; zinc finger FYVE-type containing 1; zinc finger protein, subfamily 2A (FYVE domain containing), 1; zinc finger protein, subfamily 2A, member 1; zinc finger, FYVE domain containing 1; ZNFN2A1
