Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr232 of human Aurora B.
Background
Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C (1,2). Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression (3). Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation (4,5); research investigators have observed overexpression of these kinases in a variety of human cancers (2,4). Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines (6).
Alternate Names
AIE2; AIK; AIK2; AIK3; AIM-1; AIM1; AIRK1; AIRK2; AIRK3; ARK-1; ARK-2; ARK-3; ARK1; ARK2; ARK3; AURA; AurB; AurC; AURKA; AURKB; aurkb-sv1; aurkb-sv2; AURKC; Aurora 1; Aurora 2; Aurora 3; Aurora A; Aurora B; Aurora C; Aurora kinase A; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora kinase C; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-C; Aurora-related kinase 1; Aurora-related kinase 2; Aurora-related kinase 3; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora/IPL1-related kinase 2; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 2; AURORA2; AYK1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; epididymis secretory protein Li 90; hARK1; HEL-S-90; IAK1; IPL1; IPL1-related kinase; MGC34538; PPP1R47; PPP1R48; protein phosphatase 1, regulatory subunit 47; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase aurora-B; Serine/threonine-protein kinase aurora-C; SPGF5; STK-1; STK1; STK12; STK13; STK15; STK5; STK6; STK7
