Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
ATPIF1 Antibody recognizes endogenous levels of total ATPIF1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala37 of human ATPIF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The ATPase inhibitor factor 1 (ATPIF1) gene encodes a mitochondrial ATPase inhibitor that limits ATP depletion when mitochondrial respiration is impaired (1). ATPIF1 becomes activated following a drop in pH, binding to β-F1-ATPase, thereby inhibiting the hydrolase activity of the H -ATP synthase (1,2). In addition to its role as an ATP hydrolase, ATPIF1 has also been shown to play a regulatory role in cellular energy metabolism by triggering the induction of aerobic glycolysis in cancer cells resulting in their Warburg phenotype (3,4). Research studies demonstrate that the overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis (3).
Alternate Names
ATIF1; ATP synthase F1 subunit epsilon; ATP synthase inhibitor protein; ATP synthase inhibitory factor subunit 1; ATP5IF1; ATPase inhibitor protein; ATPase inhibitor, mitochondrial; ATPase inhibitory factor 1; ATPI; ATPIF1; ATPIP; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase; IP; MGC1167; MGC8898
