Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Annexin V Antibody recognizes endogenous levels of total annexin V protein. This antibody is not predicted to cross-react with other annexin family members.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human annexin V protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Annexin V, also known as PAP-1 or Lipocortin V, is an ~30 kDa protein that binds to phospholipids in a calcium-dependent manner (1). All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2). Via direct interaction with VEGFR-2 receptor, annexin V may also signal to regulate vascular endothelial cell proliferation (3). Annexin V preferentially binds phosphatidylserine in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6). The ability of annexin V to bind specifically and robustly to phosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7). Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8). It is thought to block the production of inflammation mediators, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8).
Alternate Names
Anchorin CII; Annexin A5; Annexin V; Annexin-5; ANX5; anxa 5; ANXA5; Calphobindin I; CBP-I; Endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; RPRGL3; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha
