Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
PRP4K (D27A1) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRP4K protein.
Species Reactivity: Human, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys659 of human PRP4K protein.
Background
PRP4K (PRP4 kinase) belongs to the Clk (Cdc-like kinase) family that recognizes serine/arginine-rich substrates (1). PRP4K is involved in pre-mRNA splicing and transcriptional regulation by interacting with components of both the U5 snRNP and the NCoR Deacetylase complexes (2,3). PRP6 and PRP31 have been shown to be direct substrates of PRP4K; their phosphorylation by PRP4K contributes to the assembly of a stable spliceosomal B complex (4).
Alternate Names
dJ1013A10.1; dJ1013A10.1 (PRP4 protein kinase homolog); KIAA0536; PR4H; pre-mRNA processing factor 4B; protein-serine/threonine kinase; PRP4; PRP4 kinase; PRP4 pre-mRNA processing factor 4 homolog B; PRP4 pre-mRNA processing factor 4 homolog B (yeast); PRP4 pre-mRNA-processing factor 4 homolog; PRP4B; PRP4H; PRP4K; PRPF4B; Serine/threonine-protein kinase PRP4 homolog; serine/threonine-protein kinase PRP4K
