CST, 86397S, QKI Antibody

Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
QKI Antibody recognizes endogenous levels of total QKI protein. This antibody detects QKI-5 and other isoforms.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly202 of human QKI protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Quaking (QKI) is a member of the signal transduction and activator of RNA (STAR) protein family of RNA-binding proteins (1,2). Mutations in the mouse Qki locus results in impaired myelin formation resulting in tremors (3). QKI proteins exist as homodimers, and disruption of the dimerization process is lethal in mice (2). QKI exists in different isoforms that differ in their C-terminus, resulting in unique subcellular localizations (4). The nuclear isoform of QKI, QKI-5, is involved in regulation of alternative splicing of MAG mRNA, which encodes for a protein important for myelin sheath formation and maintenance (4,5). QKI has also been implicated in schizophrenia and oligodendrocyte differentiation (6). QKI can function as a tumor suppressor, as it is regulated by p53 to stabilize miRNAs that regulate TGF-β signaling (7).
Alternate Names
DKFZp586I0923; HKQ; homolog of mouse quaking QKI (KH domain RNA binding protein); Hqk; HqkI; KH domain-containing RNA-binding protein QKI; Protein quaking; QK; QK1; QK3; QKI; QKI, KH domain containing RNA binding; QKI/LOC100132735 fusion; quaking homolog, KH domain RNA binding; quaking homolog, KH domain RNA binding (mouse); RNA binding protein HQK