Description
Product Usage Information
For more information about the RBP-eCLIP service please visit Eclipsebio .
Western Blotting: 1:1000
Immunoprecipitation: 1:50
eCLIP: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
PRK2 (D10A10) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRK2 protein. This antibody does not cross-react with PRK1.
Species Reactivity: Human, Monkey, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu633 of human PRK2 protein.
Background
The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids (7,8). Activation and involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).
Alternate Names
cardiolipin-activated protein kinase Pak2; MGC150606; MGC71074; Pak-2; PAK2; PKN gamma; PKN2; PRK2; PRKCL2; PRO2042; Protein kinase C-like 2; protein kinase N2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2; STK7
