Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen)
Specificity / Sensitivity
CD103/ITGAE (F9M2R) Rabbit Monoclonal Antibody recognizes transfected levels of total CD103/ITGAE protein by western blot and endogenous levels of total CD103/ITGAE protein by immunofluorescence.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1158 of mouse CD103/ITGAE protein.
Background
Cluster of differentiation molecule 103 (CD103)/Integrin, alpha E (ITGAE) is a transmembrane protein forming heterodimers that are composed of α and β subunits. CD103/ITGAE is expressed by subsets of CD4 and CD8 T cells, dendritic cells, and mast cells in mucosal tissues (1,2). The heterodimer composed of CD103/ITGAE and integrin beta 7 interacts with E-cadherin, a cellular adhesion molecule found on epithelial cells. The CD103/ITGAE and integrin beta 7 complex plays a role in thymocyte proliferation, intestinal T cell homing, and the antitumor immune response (3-5). Studies have shown that the presence of CD103/ITGAE(+) immune cells can be used as markers for a favorable prognosis in different epithelial cancers (6,7).
Alternate Names
A530055J10; alph; alpha-E1; alpha-M290; aM290; CD103; integrin alpha E, epithelial-associated; Integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; ITAE; Itgae; MGC183601; OTTMUSP00000006421; OTTMUSP00000006422
