Description
Product Usage Information
IHC Leica Bond: 1:50 - 1:200
Immunohistochemistry (Paraffin): 1:50 - 1:200
Storage
Supplied in a PBS-based buffer with ~1% BSA and less than 0.1% sodium azide. Stable for 12 months when stored at 4°C. Do not aliquot the antibody . Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.
Protocol
Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
p40 (BC28) Mouse Monoclonal Antibody recognizes endogenous levels of p40 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids 5-17 of human p40 protein.
Background
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a truncated ÎNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4).
Alternate Names
AIS; amplified in squamous cell carcinoma; B(p51A); B(p51B); Chronic ulcerative stomatitis protein; CUSP; DeltaNp63; EEC3; Keratinocyte transcription factor KET; KET; LMS; NBP; OFC8; p40; p51; p53CP; P63; P73H; P73L; RHS; SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; Tumor protein 63; tumor protein p53-competing protein; tumor protein p53-like; tumor protein p63; tumor protein p63 deltaN isoform delta; Tumor protein p73-like
