Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
FoxC1 (D8A6) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxC1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr64 of human FoxC1 protein.
Background
Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA-binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4). FoxC1 (FKHL7, FREAC3) is one of two mammalian FoxC subfamily members. Along with FoxC2, it is expressed in paraxial mesoderm where it functions to promote somitogenesis, myogenesis, and vascular development, possibly under Wnt/β-catenin regulation (5). Mutations in FoxC1 are implicated in anterior segment dysgenesis (ASD) disorders, including congenital glaucoma and Axenfeld-Rieger syndrome (6). Research studies have shown that alterations in FoxC1 expression are linked to breast cancer invasiveness (7,8) and have been shown to modulate proliferation and migration of breast cancer cells (9).
Alternate Names
ARA; ASGD3; FKHL7; forkhead box C1; forkhead box C1 protein; Forkhead box protein C1; forkhead-related activator 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; forkhead, drosophila, homolog-like 7; forkhead/winged helix-like transcription factor 7; FOXC1; FREAC-3; FREAC3; IGDA; IHG1; IRID1; myeloid factor-delta; RIEG3
