Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
DYRK1A (D30C10) Rabbit Monoclonal Antibody recognizes endogenous levels of total DYRK1A protein. This antibody may also recognize a background band at ~65 kDa or ~28 kDa in some cell lines.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn523 of human DYRK1A protein.
Background
The DYRK family includes several ual-specificity t rosine-phosphorylated and egulated inases capable of phosphorylating proteins at both Tyr and Ser/Thr residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop; phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) is necessary for kinase activity. DYRKs typically autophosphorylate the Tyr residue within their activation loop, but phosphorylate substrates at Ser and Thr residues (1,6). DYRK1A phosphorylates serine and threonine residues within an RPX(S/T)P consensus sequence. Substrates include transcription factors, such as FoxO1 and cAMP response element-binding proteins, such as NFAT (7,8). DYRK1A is ubiquitously expressed in fetal and adult tissues. Transgenic mice with multiple copies of DYRK1A exhibit learning and motor defects, suggesting that it is a dosage-sensitive gene (9). The DYRK1A gene localizes to chromosome 21q22.2, a region implicated by research studies in Down syndrome, and may contribute to pathological traits observed in chromosome 21 trisomy (10).
Alternate Names
dual specificity tyrosine phosphorylation regulated kinase 1A; dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK1-related kinase; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; DYR1A; DYRK; DYRK1; DYRK1A; hMNB; HP86; MNB; mnb protein kinase homolog hp86; MNB/DYRK protein kinase; MNBH; MRD7; Protein kinase minibrain homolog; serine/threonine kinase MNB; serine/threonine-specific protein kinase
