Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 60876 .
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
NKX2.5 (E1Y8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total NKX2.5 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human NKX2.5 protein.
Background
NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Mutations in are likewise associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (3,4). Transcriptional activation of is also associated with some B and T cell leukemias that result from chromosomal translocation (5-8).
Alternate Names
Cardiac-specific homeobox; cardiac-specific homeobox 1; CHNG5; CSX; CSX1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; HLHS2; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 homeobox 5; NK2 transcription factor related, locus 5; NK2 transcription factor related, locus 5 (Drosophila); NKX 2-5; NKX2-5; NKX2.5; NKX25; NKX2E; NKX4-1; tinman homolog; tinman paralog; VSD3
