Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Phospho-PINK1 (Ser228) (E9K3K) Rabbit Monoclonal Antibody recognizes transfected levels of PINK1 protein only when phosphorylated at Ser228. Non-specific bands of unknown origin are observed migrating at ~32 kDa, and at ~80 kDa in mouse cell lines. Human species reactivity is weak by western blot.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser228 of human PINK1 protein.
Background
PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria (1-3). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (2). Research studies have shown that mutations in are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction (1,3). It has been shown that autophosphorylation of PINK1 at Ser228 and Ser402 is necessary for Parkin recruitment to damaged mitochondria (4).
Alternate Names
BRPK; FLJ27236; PARK6; PINK1; protein kinase BRPK; PTEN induced kinase 1; PTEN induced putative kinase 1; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial
