Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
SLP-2 (D2I9X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLP-2 protein. Bands of unknown identity are detected by western blot at 80 kDa.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val182 of human SLP-2 protein.
Background
Stomatin-like protein 2 (SLP-2 and also known as STOML2) is a lipid-anchored mitochondrial protein that is part of a large protein complex that regulates mitochondrial biogenesis and function. Proteomic studies identified SLP-2 as a widely expressed mitochondria-enriched protein (1). As a member of both the stomatin family and stomatin-prohibitin-flotillin-HfLC/K (SPFH) superfamily of proteins, SLP-2 forms large hetero-oligomeric complexes with other mitochondrial proteins, including prohibtin, mitofusin 2, and cardiolipin (2, 3). SLP-2 contains a highly conserved SPFH domain that mediates its ability to associate with the mitochondrial inner membrane and form specialized membrane microdomains. As an inner membrane organizer of other mitochondrial proteins, SLP-2 performs multiple mitochondrial functions, including regulation of mitochondrial biogenesis, energy/calcium homeostasis, translation, and mitochondrial-mediated cellular stress responses (3, 4, 5, 6, 7, 8). Enhanced SLP-2 expression is also associated with several human cancers, including gallbladder, rectal, and gastric cancer (9, 10, 11).
Alternate Names
EPB72-like 2; EPB72-like protein 2; HSPC108; Paraprotein target 7; Paratarg-7; SLP-2; SLP2; STML2; stomatin (EPB72)-like 2; stomatin like 2; stomatin-like 2; Stomatin-like protein 2; Stomatin-like protein 2, mitochondrial; STOML2
