Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (Frozen): 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75760 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)
Specificity / Sensitivity
CEND1 (D6A6) Rabbit Monoclonal Antibody recognizes endogenous levels of total CEND1 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CEND1 protein.
Background
The progression of progenitor cells towards neuronal differentiation is regulated by cell cycle control and the transition from proliferative to neurogenic cell divisions. Cell cycle exit and neuronal differentiation 1 (CEND1) is a neuronal protein widely expressed in the adult nervous system (1). It is implicated in the synchronization of cell cycle exit and differentiation of neuronal precursors in the developing nervous system, and its expression marks the exit of proliferative cells from the cell cycle (2,3). Levels of CEND1 expression in the subventricular zone of the adult nervous system are critical for cell cycle control and neuronal differentiation mechanisms during neonatal SVZ neurogenesis (4). It has recently been shown that neural progenitor cells (NPCs) that overexpress CEND1 display increased neuronal differentiation in a mouse model of brain injury, suggesting its potential use as a therapeutic intervention for neurodegenerative diseases and brain injury (5).
Alternate Names
BM88; BM88 antigen; cell cycle exit and neuronal differentiation 1; Cell cycle exit and neuronal differentiation protein 1; CEND; CEND1; FLJ90066; MGC34326
