Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Frozen): 1:200 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 42786 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)
Specificity / Sensitivity
GAP43 (D9C8) Rabbit Monoclonal Antibody recognizes endogenous levels of total GAP43 protein. Low levels of non-specific nuclear labeling may be observed by immunocytochemistry (ICC).
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys30 of human GAP43 protein.
Background
GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7).
Alternate Names
Axonal membrane protein GAP-43; B-50; calmodulin-binding protein P-57; GAP-43; GAP43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; NEUM; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; pp46; protein F1
