Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100 - 1:400
Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87786 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
LAMTOR1/C11orf59 (D11H6) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMTOR1/C11orf59 protein. This antibody may cross-react with LAMTOR2/ROBLD3 and LAMTOR3/MAPKSP1.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein.
Background
mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5).
Alternate Names
C11orf59; chromosome 11 open reading frame 59; CK059; FLJ20625; LAMTOR1; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; late endosomal/lysosomal adaptor, MAPK and MTOR activator 1; Lipid raft adaptor protein p18; LTOR1; p18; p27kip1 releasing factor from RhoA; p27Kip1-releasing factor from RhoA; p27RF-Rho; PDRO; Protein associated with DRMs and endosomes; Ragulator complex protein LAMTOR1; ragulator complex protein PDRO; Ragulator1; RhoA activator C11orf59
