Description
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Chromatin IP: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP
Specificity / Sensitivity
OCA-T1 (E4A8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total OCA-T1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro150 of human OCA-T1 protein.
Background
OCA-T1 is encoded by the gene ( ), which was identified in a single-cell RNA sequencing study to be expressed selectively in tuft cells from mouse and human tissues (1). The expression of OCA-T1 was observed to occur in a pattern strikingly similar to that of POU2F3, a master regulator of tuft cell development whose expression also defines a tuft cell-like variant (SCLC-P) of small cell lung cancers (2). Although containing no recognizable structural domains, OCA-T1 showed minor sequence similarity with OCA-B, a protein encoded by the gene and reported to be an activator of selected Class II POU domain transcription factors (3). Functional studies revealed that OCA-T1 forms a protein complex with POU2F3; these proteins furthermore share genomic binding sites and promote the expression of genes directly associated with tuft cell development. Lastly, Crispr/Cas9-mediated deletion of in mice revealed that OCA-T1 is functionally required for normal tuft cell development (1). Collectively, these findings suggest that OCA-T1 is a critical component of the POU2F3-containing transcriptional complex that regulates the expression of genes governing tuft cell development.
Alternate Names
C11orf53; CK053; MGC50104; OCA-T1; POU class 2 homeobox associating factor 2; POU class 2 homeobox-associating factor 2; POU domain class 2-associating factor 2
