Description
Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:1600 - 1:6400 should yield acceptable results for immunofluorescent assays.
Storage
Supplied lyophilized with a formulation of 0.01 M sodium phosphate, 0.25 M sodium chloride (pH 7.6), 15 mg/mL BSA, and 0.05% azide at 4ºC. Reconstitute lyophilized material with 0.4 mL dH2O and centrifuge until clear. The product is stable for six weeks at 4ºC as an undiluted liquid. For extended storage, aliquot and freeze at -80ºC, or add an equal volume of glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles. Protect from light.
Specificity / Sensitivity
Goat Anti-Mouse IgG2a, Fc gamma Specific Antibody (Alexa Fluor
Species Reactivity: Mouse
Background
This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.
